Slide #DMS 158B [Human; pituitary gland, Pearse trichrome].

This section of pituitary is stained with a trichrome stain that nicely highlights the three different endocrine cell populations of the adenohypophysis. With this stain, acidophils are stained a yellow-orange color, basophils a reddish color, and the chromophobes are unstained. Note the great abundance of acidophils which account for about 70% of the cells in the anterior lobe.

A high power view of this trichrome-stained adenohypophysis allows for easy identification of acidophils, basophils and chromophobes.